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A novel diagnostic approach to detecting porcine epidemic diarrhea virus: The lateral immunochromatography assay

Kim, Yong Kwan, Lim, Seong-In, Cho, In-Soo, Cheong, Kwang-Myun, Lee, Eun-Jeong, Lee, Sang-Oh, Kim, Joon-Bae, Kim, Jung-Hwa, Jeong, Dong-Soo, An, Byung-Hyun, An, Dong-Jun
Journal of virological methods 2015 v.225 pp. 4-8
Porcine epidemic diarrhea virus, RNA-directed DNA polymerase, ambient temperature, antigens, diagnostic techniques, diarrhea, farms, genes, immunoaffinity chromatography, mortality, nucleocapsid, pathogens, piglets, quantitative polymerase chain reaction, reverse transcriptase polymerase chain reaction, storage temperature
Porcine epidemic diarrhea virus (PEDV) causes acute diarrhea and dehydration in sucking piglets and has a high mortality rate. An immunochromatography (IC) assay, known as a lateral flow test, is a simple device intended to detect the presence of target pathogens. Here, we developed an IC assay that detected PEDV antigens with 96.0% (218/227) sensitivity and 98.5% (262/266) specificity when compared with real-time reverse transcriptase (RT)-PCR using FAM-labeled probes based on sequences from nucleocapsid genes. The detection limits of the real-time RT-PCR and IC assays were 1×10² and 1×10³ copies, respectively. The IC assay developed herein did not detect non-specific reactions with other viral or bacterial pathogens, and the assay could be stored at 4°C or room temperature for 15 months without affecting its efficacy. Thus, the IC assay may result in improved PED detection and control on farms, and is a viable alternative to current diagnostic tools for PEDV.