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A novel diagnostic approach to detecting porcine epidemic diarrhea virus: The lateral immunochromatography assay

Author:
Kim, Yong Kwan, Lim, Seong-In, Cho, In-Soo, Cheong, Kwang-Myun, Lee, Eun-Jeong, Lee, Sang-Oh, Kim, Joon-Bae, Kim, Jung-Hwa, Jeong, Dong-Soo, An, Byung-Hyun, An, Dong-Jun
Source:
Journal of virological methods 2015 v.225 pp. 4-8
ISSN:
0166-0934
Subject:
Porcine epidemic diarrhea virus, RNA-directed DNA polymerase, ambient temperature, antigens, diagnostic techniques, diarrhea, farms, genes, immunoaffinity chromatography, mortality, nucleocapsid, pathogens, piglets, quantitative polymerase chain reaction, reverse transcriptase polymerase chain reaction, storage temperature
Abstract:
Porcine epidemic diarrhea virus (PEDV) causes acute diarrhea and dehydration in sucking piglets and has a high mortality rate. An immunochromatography (IC) assay, known as a lateral flow test, is a simple device intended to detect the presence of target pathogens. Here, we developed an IC assay that detected PEDV antigens with 96.0% (218/227) sensitivity and 98.5% (262/266) specificity when compared with real-time reverse transcriptase (RT)-PCR using FAM-labeled probes based on sequences from nucleocapsid genes. The detection limits of the real-time RT-PCR and IC assays were 1×10² and 1×10³ copies, respectively. The IC assay developed herein did not detect non-specific reactions with other viral or bacterial pathogens, and the assay could be stored at 4°C or room temperature for 15 months without affecting its efficacy. Thus, the IC assay may result in improved PED detection and control on farms, and is a viable alternative to current diagnostic tools for PEDV.
Agid:
6026827