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Selected microbial degradation of saturated methyl branched-chain fatty acid isomers
- Ngo, Helen L., Ashby, Richard D., Nunez, Alberto
- Journal of the American Oil Chemists' Society 2012 v.89 no.10 pp. 1885
- Pseudomonas, bacteria, biodegradation, branched chain fatty acids, carbon, feedstocks, gas chromatography, isomers, microbial growth, spectroscopy
- Three strains of Pseudomonas bacteria were screened for their capabilities of degrading chemically synthesized saturated branched-chain fatty acids (sbc-FAs). Mixtures of sbc-FAs with the methyl-branch located at various locales along the fatty acid were used as a carbon feedstock in shake-flask culture. Utilization (and hence degradability) of the sbc-FAs was monitored based on positive bacterial growth, fatty acid recovery rates and chromatographic (GC and GC-MS) analysis of the recovered carbon source. Pseudomonas (P.) putida KT2442 and P. oleovorans NRRL B-14683 were both able to grow on sbc-FAs utilizing 35% and 27% of the carbon source, respectively after 144 h. In contrast, P. resinovorans NRRL B-2649 exhibited the most efficient use of the carbon source by utilizing 89% of the starting material after 96 h resulting in a cell dry weight (CDW) of 3.1 g/L. GC and GC-MS analysis of the recovered carbon source revealed that the bacterial strains selectively utilized the isostearic acid in the sbc-FA mixture and a new group of short chain fatty acids (approximately 4 to 29%) were formed during degradation.