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Transgenic expression of an insect diapause-specific peptide (DSP) in Arabidopsis resists phytopathogenic fungal attacks

Wu, Tingquan, Chen, Yongfang, Chen, Weida, Zou, Shenghao, Zhang, Yongqing, Lin, Yu’e, Liang, Zhaojun, Tang, Dingzhong
European journal of plant pathology 2013 v.137 no.1 pp. 93-101
Arabidopsis, Botrytis cinerea, Fusarium solani, Gastrophysa, Trichophyton rubrum, adults, animal pathogenic fungi, antifungal properties, crops, gene expression, gene fusion, insects, plant pathogenic fungi, transgenic plants
Diapause-specific peptide (DSP) is an insect antimicrobial peptide, composed of 41 amino acid residues including six cysteines and isolated from diapausing adults of the leaf beetle Gastrophysa atrocyaneais. Recent research results have been demonstrated that in vitro, DSP has selective antifungal activity against the higher animal pathogenic fungus Trichophyton rubrum and the plant phytopathogenic fungus Fusarium solani. To elucidate the effect of DSP on other phytopathogenic fungi, we respectively introduced a Cht1SP-DSP-FLAG fusion gene and Cht1SP-FLAG fusion gene into Arabidopsis ecotype col-0. Transgenic plants expressing Cht1SP-DSP-FLAG fusion gene showed significant resistance to Golovinomyces cichoracearum (biotrophic fungus), Botrytis cinerea (necrotrophic fungus) and Hyaloperonospora arabidopsidis (H. a.) Noco2 (oomycete), whilst transgenic plants expressing Cht1SP-FLAG fusion gene showed no resistance to these pathogens. These results indicated that for many plant pathogenic fungi, DSP has high antimicrobial activity, which suggests that DSP has a huge potential in protecting crops from damage from phytopathogenic fungi.