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An OFF–ON Two-Photon Fluorescent Probe for Tracking Cell Senescence in Vivo

Author:
Lozano-Torres, Beatriz, Galiana, Irene, Rovira, Miguel, Garrido, Eva, Chaib, Selim, Bernardos, Andrea, Muñoz-Espín, Daniel, Serrano, Manuel, Martínez-Máñez, Ramón, Sancenón, Félix
Source:
Journal of the American Chemical Society 2017 v.139 no.26 pp. 8808-8811
ISSN:
1520-5126
Subject:
cell senescence, drug therapy, emissions factor, fluorescence, fluorescent dyes, galactose, histidine, mice, nitrogen
Abstract:
A naphthalimide-based two-photon probe (AHGa) for the detection of cell senescence is designed. The probe contains a naphthalimide core, an l-histidine methyl ester linker, and an acetylated galactose bonded to one of the aromatic nitrogen atoms of the l-histidine through a hydrolyzable N-glycosidic bond. Probe AHGa is transformed into AH in senescent cells resulting in an enhanced fluorescent emission intensity. In vivo detection of senescence is validated in mice bearing tumor xenografts treated with senescence-inducing chemotherapy.
Agid:
6031145