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Physico-chemical characterization of protein-associated polysaccharides extracted from sugar beet pulp
- Marshall L. Fishman, Hoa K. Chau, Phoebe X. Qi, Arland T. Hotchkiss, Madhav P. Yadav
- Carbohydrate polymers 2013 v.92 no.2 pp. 2257-2266
- pectins, viscosity, steam, crude protein, sugar beet pulp, polymers, molecular weight, solubilization, carboxymethylcellulose
- We have solubilized and separated polysaccharides from sugar beet pulp (SBP) into three fractions with steam assisted flash extraction (SAFE). For pectin, recovery ranged from 8 to 14%, degree of methy-esterification 66–73%, crude protein 1.3–1.7%, Mw 262–318kDa, ηw 0.22–0.23dL/g, Rgz 36–39nm and Rhz 41–42nm. For alkaline soluble polysaccharides, (ASP I) recovery ranged from 4.0 to 6.5%, crude protein 1.2–4.8%, weight average molar mass (Mw) 66–68kDa, weight average intrinsic viscosity (ηw) 0.27–0.30dL/g, z-average radius of gyration (Rgz) 25–29nm and z-average hydrated radius (Rhz) 10–11nm. ASP II recovery ranged from 2.0 to 8.6%, crude protein 1.2–4.8%, Mw 299–339kDa, ηw 0.22–0.33dL/g, Rgz 33–34nm and Rhz 30–34nm. Recovery of the residue mainly cellulose, ranged from 20.3 to 22.3%. The cellulose in this fraction was converted to carboxymethyl cellulose (CMC). The CMC fraction contained 0.33–0.43 crude protein and had an Mw ranging from 127 to 263kDa, ηw 3.6–8.0dL/g, Rgz 35–45nm and Rhz 27–40nm.