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Physico-chemical characterization of protein-associated polysaccharides extracted from sugar beet pulp

Marshall L. Fishman, Hoa K. Chau, Phoebe X. Qi, Arland T. Hotchkiss, Madhav P. Yadav
Carbohydrate polymers 2013 v.92 no.2 pp. 2257-2266
pectins, viscosity, steam, crude protein, sugar beet pulp, polymers, molecular weight, solubilization, carboxymethylcellulose
We have solubilized and separated polysaccharides from sugar beet pulp (SBP) into three fractions with steam assisted flash extraction (SAFE). For pectin, recovery ranged from 8 to 14%, degree of methy-esterification 66–73%, crude protein 1.3–1.7%, Mw 262–318kDa, ηw 0.22–0.23dL/g, Rgz 36–39nm and Rhz 41–42nm. For alkaline soluble polysaccharides, (ASP I) recovery ranged from 4.0 to 6.5%, crude protein 1.2–4.8%, weight average molar mass (Mw) 66–68kDa, weight average intrinsic viscosity (ηw) 0.27–0.30dL/g, z-average radius of gyration (Rgz) 25–29nm and z-average hydrated radius (Rhz) 10–11nm. ASP II recovery ranged from 2.0 to 8.6%, crude protein 1.2–4.8%, Mw 299–339kDa, ηw 0.22–0.33dL/g, Rgz 33–34nm and Rhz 30–34nm. Recovery of the residue mainly cellulose, ranged from 20.3 to 22.3%. The cellulose in this fraction was converted to carboxymethyl cellulose (CMC). The CMC fraction contained 0.33–0.43 crude protein and had an Mw ranging from 127 to 263kDa, ηw 3.6–8.0dL/g, Rgz 35–45nm and Rhz 27–40nm.