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Grapevine VpPR10.1 functions in resistance to Plasmopara viticola through triggering a cell death‐like defence response by interacting with VpVDAC3

Ma, Hui, Xiang, Gaoqing, Li, Zhiqian, Wang, Yuting, Dou, Mengru, Su, Li, Yin, Xiao, Liu, Ruiqi, Wang, Yuejin, Xu, Yan
Plant biotechnology journal 2018 v.16 no.8 pp. 1488-1501
Nicotiana benthamiana, Plasmopara viticola, Vitis, cell death, cultivars, downy mildew, genetic improvement, genetically modified organisms, germplasm, grapes, pathogens, precipitin tests, two hybrid system techniques
As one of the most serious diseases in grape, downy mildew caused by Plasmopara viticola is a worldwide grape disease. Much effort has been focused on improving susceptible grapevine resistance, and wild resistant grapevine species are important for germplasm improvement of commercial cultivars. Using yeast two‐hybrid screen followed by a series of immunoprecipitation experiments, we identified voltage‐dependent anion channel 3 (VDAC3) protein from Vitis piasezkii ‘Liuba‐8’ as an interacting partner of VpPR10.1 cloned from Vitis pseudoreticulata ‘Baihe‐35‐1’, which is an important germplasm for its resistance to a range of pathogens. Co‐expression of VpPR10.1/VpVDAC3 induced cell death in Nicotiana benthamiana, which accompanied by ROS accumulation. VpPR10.1 transgenic grapevine line showed resistance to P. viticola. We conclude that the VpPR10.1/VpVDAC3 complex is responsible for cell death‐mediated defence response to P. viticola in grapevine.