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Development and Evaluation of Monoclonal Antibodies for the Glucoside of T-2 Toxin (T2-Glc)

Author:
Maragos, Chris M., Kurtzman, Cletus, Busman, Mark, Price, Neil, McCormick, Susan
Source:
Toxins 2013 v.5 no.7 pp. 1299
ISSN:
2072-6651
Subject:
HT-2 toxin, T-2 toxin, analytical methods, clones, cross reaction, fungi, glucosides, hybridomas, immunoassays, inhibitory concentration 50, metabolites, mice, monoclonal antibodies, plants (botany), toxicity
Abstract:
The interactions between fungi and plants can yield metabolites that are toxic in animal systems. Certain fungi are known to produce sesquiterpenoid trichothecenes, such as T-2 toxin, that are biotransformed by several mechanisms including glucosylation. The glucosylated forms have been found in grain and are of interest as potential reservoirs of T-2 toxin that are not detected by many analytical methods. Hence the glucosides of trichothecenes are often termed “masked” mycotoxins. The glucoside of T-2 toxin (T2-Glc) was linked to keyhole limpet hemocyanin and used to produce antibodies in mice. Ten monoclonal antibody (Mab)-producing hybridoma cell lines were developed. The Mabs were used in immunoassays to detect T2-Glc and T-2 toxin, with midpoints of inhibition curves (IC50s) in the low ng/mL range. Most of the Mabs demonstrated good cross-reactivity to T-2 toxin, with lower recognition of HT-2 toxin. One of the clones (2-13) was further characterized with in-depth cross-reactivity and solvent tolerance studies. Results suggest Mab 2-13 will be useful for the simultaneous detection of T-2 toxin and T2-Glc.
Agid:
60329
Handle:
10113/60329