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A mitochondrial targeted two-photon iridium(III) phosphorescent probe for selective detection of hypochlorite in live cells and in vivo

Author:
Li, Guanying, Lin, Qian, Sun, Lingli, Feng, Changsheng, Zhang, Pingyu, Yu, Bole, Chen, Yu, Wen, Ya, Wang, Hui, Ji, Liangnian, Chao, Hui
Source:
Biomaterials 2015 v.53 pp. 285-295
ISSN:
0142-9612
Subject:
Danio rerio, absorption, chlorides, endotoxins, hydrogen peroxide, image analysis, ions, iridium, lipopolysaccharides, liver, mitochondria, moieties, myeloperoxidase, oxidation, phosphorescence
Abstract:
Endogenous hypochlorite ion (ClO⁻) is a highly reactive oxygen species (ROS) that is produced from hydrogen peroxide and chloride ions catalyzed by myeloperoxidase (MPO). And mitochondrion is one of the major sources of ROS including ClO⁻. In the present work, a two-photon phosphorescent probe for ClO⁻ in mitochondria was developed. An iridium(III) complex bearing a diaminomaleonitrile group as ClO⁻ reactive moiety specifically responded to ClO⁻ over other ions and ROSs. When the probe was reacted with ClO⁻ to form an oxidized carboxylate product, a significant enhancement in phosphorescence intensity was observed under one-photon (402 nm) and two-photon (750 nm) excitation, with a two-photon absorption cross-section of 78.1 GM at 750 nm. More importantly, ICP-MS results and cellular images co-stained with Mito-tracker Green demonstrated that this probe possessed high specificity for mitochondria. This probe was applied in the one- and two-photon imaging of ClO⁻ in vitro and in vivo. The results suggested endotoxin lipopolysaccharide (LPS) induced ClO⁻ mostly generated in the liver of zebrafish.
Agid:
6034938