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Arginine-rich polyplexes for gene delivery to neuronal cells

Author:
Morris, Viola B., Labhasetwar, Vinod
Source:
Biomaterials 2015 v.60 pp. 151-160
ISSN:
0142-9612
Subject:
arginine, astrocytes, biocompatibility, blood-brain barrier, brain, confocal microscopy, erythrocytes, gene expression, gene therapy, genes, image analysis, immunohistochemistry, intravenous injection, mice, nervous system diseases, neurons, oligopeptides, plasmids, polyethylene glycol, polyethyleneimine, rats, transfection
Abstract:
Neuronal gene therapy potentially offers an effective therapeutic intervention to cure or slow the progression of neurological diseases. However, neuronal cells are difficult to transfect with nonviral vectors, and in vivo their transport across the blood–brain barrier (BBB) is inefficient. We synthesized a series of arginine-rich oligopeptides, grafted with polyethyleneimine (PEI) and modified with a short-chain polyethylene glycol (PEG). We hypothesized that the arginine would enhance cellular uptake and transport of these polyplexes across the BBB, with PEG imparting biocompatibility and “stealth” properties and PEI facilitating DNA condensation and gene transfection. The optimized composition of the polyplexes demonstrated hemocompatibility with red blood cells, causing no lysis or aggregation, and showed significantly better cytocompatibility than PEI in vitro. Polyplexes formulated with luciferase-expressing plasmid DNA could transfect rat primary astrocytes and neurons in vitro. Confocal imaging data showed efficient cellular uptake of DNA and its sustained intracellular retention and nuclear localization with polyplexes. Intravenous administration of the optimized polyplexes in mice led to gene expression in the brain, which upon further immunohistochemical analysis demonstrated gene expression in neurons. In conclusion, we have successfully designed a nonviral vector for in vitro and in vivo neuronal gene delivery.
Agid:
6035067