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Recombinant production of a chimeric antimicrobial peptide in E. coli and assessment of its activity against some avian clinically isolated pathogens

Author:
Tanhaiean, Abass, Azghandi, Marjan, Razmyar, Jamshid, Mohammadi, Elyas, Sekhavati, Mohammad Hadi
Source:
Microbial pathogenesis 2018 v.122 pp. 73-78
ISSN:
0882-4010
Subject:
Escherichia coli, affinity chromatography, antibacterial properties, antibiotics, antimicrobial peptides, bacteria, blood serum, camels, chickens, hemolysis, lactoferrin, multiple drug resistance, nickel, pathogens, poultry industry, veterinary medicine
Abstract:
Over the last decades, poultry industry faced to the rapid emergence of multidrug-resistant bacteria as a global concern. Antimicrobial peptide (AMPs) known as potential antibiotic alternative and were considered as a new antimicrobial agent. Current methods of production and purification of AMPs have several limitations such as: costly, time-consuming and killing the producing host cells in recombinant form. In the present study, a chimeric peptide derived from camel lactoferrin was produced in Escherichia coli periplasmic space using a pET-based expression system and its antibacterial activity was determined on some avian pathogens in vitro. A carboxy-terminal polyhistidine tag was used for purification by Ni²⁺ affinity chromatography with an average yield of 0.42 g/L. The His-tagged chimeric peptide showed different range of antimicrobial activity against clinically isolated avian pathogens with low chicken blood hemolysis activity and high serum stability. Overall, the results of this investigation showed the recombinant chimeric peptide was successfully expressed in pET-based expression system and could be considered as a proper alternative for some currently used antibiotics in poultry industry and drugs veterinary medicine.
Agid:
6037125