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Identification of chickpea (Cicer arietinum) in foods using a novel real-time polymerase chain reaction detection method
- Nakamura, Kosuke, Ishigaki, Takumi, Kobayashi, Tomoko, Kimata, Shinya, Soga, Keisuke, Fujii, Uki, Kishine, Masahiro, Takabatake, Reona, Mano, Junichi, Kitta, Kazumi, Kawakami, Hiroshi, Nishimaki-Mogami, Tomoko, Kondo, Kazunari
- Subtropical plant science 2018 v.71 pp. 8-16
- 9-cis-epoxycarotenoid dioxygenase, Cicer arietinum, Southern blotting, chickpeas, foods, genes, haploidy, quantitative polymerase chain reaction, sequence homology
- A novel real-time polymerase chain reaction (PCR) method for detecting chickpea was developed. From homologous sequences of 9-cis-epoxycarotenoid dioxygenase gene (NCED) among leguminous species, chickpea’s NCED was cloned, and the Southern-blot analysis showed that NCED is a single copy gene in a haploid genome. Its coding sequences at the 5′ terminus were found unique to chickpea and conserved among various chickpea varieties. Developed real-time PCR method targeting the unique sequences was specific to chickpea and had detection limit of approximately 30 copies per a reaction, and applicable for qualitative detection of chickpea in various forms of food products including dried, powdered, retort-packed, canned, fermented and pasted. Our results showed that the developed method enables identification of chickpea in foods.