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Inhibitors of biofilm formation by biofuel fermentation contaminants

Author:
Timothy D. Leathers, Kenneth M. Bischoff, Joseph O. Rich, Neil P.J. Price, Pennapa Manitchotpisit, Melinda S. Nunnally, Amber M. Anderson
Source:
Bioresource technology 2014 v.169 pp. 45-51
ISSN:
0960-8524
Subject:
Bacillus subtilis, Lactobacillus brevis, Lactobacillus fermentum, Lactobacillus plantarum, bacteriophages, biofilm, biofuels, citrates, endolysin, ethanol fuels, fermentation, polysaccharides, xylitol
Abstract:
Biofuel fermentation contaminants such as Lactobacillus sp. may persist in production facilities by forming recalcitrant biofilms. In this study, biofilm-forming strains of Lactobacillus brevis, Lactobacillus fermentum, and Lactobacillus plantarum were isolated and characterized from a dry-grind fuel ethanol plant. A variety of potential biofilm inhibitors were tested, including microbial polysaccharides, commercial enzymes, ferric ammonium citrate, liamocins, phage endolysin, xylitol, and culture supernatants from Bacillus sp. A commercial enzyme mixture (Novozyme 188) and culture supernatants from Bacillus subtilis strains ALT3A and RPT-82412 were identified as the most promising biofilm inhibitors. In biofilm flow cells, these inhibitors reduced the density of viable biofilm cells by 0.8–0.9logcfu/cm2. Unlike B. subtilis strain RPT-82412, B. subtilis strain ALT3A and Novozyme 188 did not inhibit planktonic growth of Lactobacillus sp. MALDI-TOF mass spectra showed the production of surfactin-like molecules by both B. subtilis strains, and the coproduction of iturin-like molecules by strain RPT-82412.
Agid:
60390
Handle:
10113/60390