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Inhibitors of biofilm formation by biofuel fermentation contaminants
- Leathers, Timothy D., Bischoff, Kenneth M., Rich, Joseph O., Price, Neil P.J., Manitchotpisit, Pennapa, Nunnally, Melinda S., Anderson, Amber M.
- Bioresource technology 2014 v.169 pp. 45
- Bacillus subtilis, Lactobacillus brevis, Lactobacillus fermentum, Lactobacillus plantarum, bacteriophages, biofilm, biofuels, citrates, endolysin, ethanol fuels, fermentation, polysaccharides, xylitol
- Biofuel fermentation contaminants such as Lactobacillus sp. may persist in production facilities by forming recalcitrant biofilms. In this study, biofilm-forming strains of Lactobacillus brevis, Lactobacillus fermentum, and Lactobacillus plantarum were isolated and characterized from a dry-grind fuel ethanol plant. A variety of potential biofilm inhibitors were tested, including microbial polysaccharides, commercial enzymes, ferric ammonium citrate, liamocins, phage endolysin, xylitol, and culture supernatants from Bacillus sp. A commercial enzyme mixture (Novozyme 188) and culture supernatants from Bacillus subtilis strains ALT3A and RPT-82412 were identified as the most promising biofilm inhibitors. In biofilm flow cells, these inhibitors reduced the density of viable biofilm cells by 0.8–0.9logcfu/cm2. Unlike B. subtilis strain RPT-82412, B. subtilis strain ALT3A and Novozyme 188 did not inhibit planktonic growth of Lactobacillus sp. MALDI-TOF mass spectra showed the production of surfactin-like molecules by both B. subtilis strains, and the coproduction of iturin-like molecules by strain RPT-82412.