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Peroxidase-mediated conjugation of corn fiber gum and bovine serum albumin to improve emulsifying properties
- Liu, Yan, Qiu, Shuang, Li, Jinlong, Chen, Hao, Tatsumi, Eizo, Yadav, Madhav, Yin, Lijun
- Carbohydrate polymers 2015 v.118 pp. 70-78
- arabinoxylan, bovine serum albumin, corn, crosslinking, emulsifiers, emulsifying properties, emulsions, food industry, ionic strength, pH, peroxidase, polymers, zeta potential
- The emulsifying properties of corn fiber gum (CFG), a naturally occurring polysaccharide–protein complex, was improved by kinetically controlled formation of hetero-covalent linkages with bovine serum albumin (BSA), using horseradish peroxidase (HRP). The formation of hetero-crosslinked CFG–BSA conjugates was confirmed using ultraviolet–visible and Fourier-transform infrared analyses. The optimum CFG–BSA conjugates were prepared at a CFG:BSA weight ratio of 10:1, and peroxidase:BSA weight ratio of 1:4000. Selected CFG–BSA conjugates were used to prepare oil-in-water emulsions; the emulsifying properties were better than those of emulsions stabilized with only CFG or BSA. Measurements of mean droplet sizes and zeta potentials showed that CFG–BSA-conjugate-stabilized emulsions were less susceptible to environmental stresses, such as pH changes, high K ionic strengths, and freeze–thaw treatments than CFG- or BSA-stabilized emulsions. These conjugates have potential applications as novel emulsifiers in food industry.