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Development and validation of an HPLC-MS/MS analytical method for quantitative analysis of TCBA-TPQ, a novel anticancer makaluvamine analog, and application in a pharmacokinetic study in rats

Author:
YU, Jun-Xian, Voruganti, Sukesh, LI, Dan-Dan, Qin, Jiang-Jiang, Nag, Subhasree, Xu, Su, Velu, Sadanandan E., Wang, Wei, ZHANG, Ruiwen
Source:
Chinese journal of natural medicines 2015 v.13 no.7 pp. 554-560
ISSN:
1875-5364
Subject:
Oriental traditional medicine, analytical methods, antineoplastic agents, humans, in vivo studies, medicinal properties, neoplasms, pharmacokinetics, quantitative analysis, rats
Abstract:
We have recently designed and synthesized several novel iminoquinone anticancer agents that have entered preclinical development for the treatment of human cancers. Herein we developed and validated a quantitative HPLC-MS/MS analytical method for one of the lead novel anticancer makaluvamine analog, TCBA-TPQ, and conducted a pharmacokinetic study in laboratory rats. Our results indicated that the HPLC-MS/MS method was precise, accurate, and specific. Using this method, we carried out in vitro and in vivo evaluations of the pharmacological properties of TCBA-TPQ and plasma pharmacokinetics in rats. Our results provide a basis for future preclinical and clinical development of this promising anticancer marine analog.
Agid:
6041909