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Profiling of the Terpene Metabolome in Carrot Fruits of Wild (Daucus carota L. ssp. carota) Accessions and Characterization of a Geraniol Synthase
- Yahyaa, Mosaab, Ibdah, Muhammad, Marzouk, Sally, Ibdah, Mwafaq
- Journal of agricultural and food chemistry 2016 v.66 no.10 pp. 2378-2386
- Daucus carota, biosynthesis, carrots, flavor compounds, fruits, genes, geraniol, metabolome, odors, oils, seeds, sesquiterpenoids
- Fruits from wild carrot (Daucus carota L. ssp. carota) have been used for medicinal purposes since ancient times. The oil of its seeds, with their abundant monoterpenes and sesquiterpenes, has drawn attention in recent years because of its potential pharmaceutical application. A combined chemical, biochemical, and molecular study was conducted to evaluate the differential accumulation of terpene volatiles in carrot fruits of wild accessions. This work reports a similarity-based cloning strategy identification and functional characterization of one carrot monoterpene terpene synthase, WtDcTPS1. Recombinant WtDcTPS1 protein produces mainly geraniol, the predominant monoterpene in carrot seeds of wild accession 23727. The results suggest a role for the WtDcTPS1 gene in the biosynthesis of carrot fruit aroma and flavor compounds.