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Ultrafast Screening of a Novel, Moderately Hydrophilic Angiotensin-Converting-Enzyme-Inhibitory Peptide, RYL, from Silkworm Pupa Using an Fe-Doped-Silkworm-Excrement-Derived Biocarbon: Waste Conversion by Waste

Author:
Liu, Long, Wei, Yanan, Chang, Qing, Sun, Huaju, Chai, Kungang, Huang, Zuqiang, Zhao, Zhenxia, Zhao, Zhongxing
Source:
Journal of agricultural and food chemistry 2017 v.65 no.51 pp. 11202-11211
ISSN:
1520-5118
Subject:
agricultural wastes, byproducts, carbon, hydrogen bonding, hydrophilicity, inhibitory concentration 50, iron, magnetic properties, peptides, pupae, screening, silkworms
Abstract:
A novel, moderately hydrophilic peptide (RYL) with high ACE-inhibitory activity was screened ultrafast via a concept of waste conversion using waste. This novel peptide was screened from silkworm pupa using an Fe-doped porous biocarbon (FL/Z-SE) derived from silkworm excrement. FL/Z-SE possessed magnetic properties and specific selection for peptides due to Fe’s dual functions. The selected RYL, which has moderate hydrophilicity (LogP = −0.22), exhibited a comparatively high ACE-inhibitory activity (IC₅₀ = 3.31 ± 0.11 μM). The inhibitory kinetics and docking-simulation results show that, as a competitive ACE inhibitor, RYL formed five hydrogen bonds with the ACE residues in the S1 and S2 pockets. In this work, both the screening carbon material and the selected ACE-inhibitory peptide were derived from agricultural waste (silkworm excrement and pupa), which offers a new way of thinking about the development of advanced uses of the silkworm byproducts and wastes.
Agid:
6042273