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Analysis of the Effects of δ-Tocopherol on RAW264.7 and K562 Cells Based on 1H NMR Metabonomics

Lu, Yang, Li, Hui, Geng, Yue
Journal of agricultural and food chemistry 2018 v.66 no.4 pp. 1039-1046
aerobiosis, anaerobiosis, betaine, bioactive properties, cell lines, delta-tocopherol, inhibitory concentration 50, lactic acid, metabolites, metabolomics, nuclear magnetic resonance spectroscopy, protein synthesis, urea cycle
δ-Tocopherol (δ-TOH) is a form of vitamin E with higher bioactivity. In this study, we studied the bioactivity of δ-TOH using the IC₅₀ of δ-TOH on RAW264.7 (80 μM) and K562 (110 μM) cells. We compared the differential metabolites from the cell lines with and without δ-TOH treatment by ¹H NMR metabonomics analysis. It was found that δ-TOH affected the protein biosynthesis, betaine metabolism, and urea cycle in various ways in both cell lines. Metabolic levels of the cell lines were changed after treatment with δ-TOH as differential metabolites were produced. The betaine level in RAW264.7 cells was reduced significantly, while the l-lactic acid level in K562 cells was significantly enhanced. The metabolic changes might contribute to the switch of the respiration pattern from aerobic respiration to anaerobic respiration in K562 cells. These results are helpful in further understanding the subtoxicity of δ-TOH.