Main content area

Neuroprotective Effect of Hydroxytyrosol in Experimental Diabetic Retinopathy: Relationship with Cardiovascular Biomarkers

González-Correa, José Antonio, Rodríguez-Pérez, María Dolores, Márquez-Estrada, Lucía, López-Villodres, Juan Antonio, Reyes, José Julio, Rodriguez-Gutierrez, Guillermo, Fernández-Bolaños, Juan, De La Cruz, José Pedro
Journal of agricultural and food chemistry 2018 v.66 no.3 pp. 637-644
biomarkers, diabetic retinopathy, extracellular matrix, ganglia, neuroprotective effect, platelet aggregation, prostacyclin, rats, regression analysis, retina
The aim of the study was to test the neuroprotective effect of hydroxytyrosol (HT) on experimental diabetic retinopathy. Animals were divided in four groups: (1) control nondiabetic rats, (2) streptozotocin-diabetic rats (DR), (3) DR treated with 1 mg/kg/day p.o. HT, and (4) DR treated with 5 mg/kg/day p.o. HT. Treatment with HT was started 7 days before inducing diabetes and was maintained for 2 months. In the DR group, total area occupied by extracellular matrix was increased, area occupied by retinal cells was decreased; both returned to near-control values in DR rats treated with HT. The number of retinal ganglion cells in DR was significantly lower (44%) than in the control group, and this decrease was smaller after HT treatment (34% and 9.1%). Linear regression analysis showed that prostacyclin, platelet aggregation, peroxynitrites, and the dose of 5 mg/kg/day HT significantly influenced retinal ganglion cell count. In conclusion, HT exerted a neuroprotective effect on diabetic retinopathy, and this effect correlated significantly with changes in some cardiovascular biomarkers.