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Development of a Sono-Assembled, Bifunctional Soy Peptide Nanoparticle for Cellular Delivery of Hydrophobic Active Cargoes
- Zhang, Yuanhong, Zhao, Mouming, Ning, Zhengxiang, Yu, Shujuan, Tang, Ning, Zhou, Feibai
- Journal of agricultural and food chemistry 2018 v.66 no.16 pp. 4208-4218
- antioxidants, bioavailability, curcumin, encapsulation, enzymes, food processing, hydrophobicity, lipid peroxidation, models, nanoparticles, particle size, proteolysis, sonication, soy protein, storage quality, sustainable development, toxicity, water solubility
- Soy proteins are prone to aggregate upon proteolysis, hindering their sustainable development in food processing. Here, a continuous work on the large insoluble peptide aggregates was carried out, aiming to develop a new type of soy peptide-based nanoparticle (SPN) for active cargo delivery. Sono-assembled SPN in spherical appearance and core–shell structure maintained by noncovalent interactions was successfully fabricated, exhibiting small particle size (103.95 nm) in a homogeneous distribution state (PDI = 0.18). Curcumin as a model cargo was efficiently encapsulated into SPN upon sonication, showing high water dispersity (129.6 mg/L, 10⁴ higher than its water solubility) and storage stability. Additionally, the pepsin-resistant SPN contributed to the controlled release of curcumin at the intestinal phase and thus significantly improved the bioaccessibility. Encapsulated curcumin was effective in protecting glutamate-induced toxicity in PC12 cells, where the matrix SPN can simultaneously reduce lipid peroxidation and elevate antioxidant enzymes levels, innovatively demonstrating its bifunctionality during cellular delivery.