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Anti-Inflammatory and Antioxidant Activities from the Basolateral Fraction of Caco-2 Cells Exposed to a Rosmarinic Acid Enriched Extract
- Villalva, Marisol, Jaime, Laura, Aguado, Estela, Nieto, Juan Antonio, Reglero, Guillermo, Santoyo, Susana
- Journal of agricultural and food chemistry 2018 v.66 no.5 pp. 1167-1174
- Origanum majorana, anti-inflammatory activity, antioxidant activity, antioxidants, bioavailability, digestion, human cell lines, humans, in vitro digestion, interleukin-1beta, interleukin-6, intestinal absorption, liquids, macrophages, mass spectrometry, models, rosmarinic acid, secretion, tumor necrosis factor-alpha
- The potential use of Origanum majorana L. as a source of bioavailable phenolic compounds, specifically rosmarinic acid (RA), has been evaluated. Phenolic bioavailability was tested using an in vitro digestion process followed by a Caco-2 cellular model of intestinal absorption. The high-performance liquid chromatography–photodiode array detector-tandem mass spectrometry (HPLC–PAD-MS/MS) analysis showed the main components in the extract were 6-hydroxyluteolin-7-O-glucoside and rosmarinic acid, followed by luteolin-O-glucoside. After digestion process, the amount of total phenolic compounds (TPC) only decreased slightly, although a remarkable reduction in RA (near 50%) was detected. Bioavailable fraction contained 7.37 ± 1.39 mg/L digested extract of RA with small quantities of lithospermic acid and diosmin and presented an important antioxidant activity (0.89 ± 0.09 mmol Trolox/L digested extract). Besides, this bioavailable fraction produced a significant inhibition in TNF-α, IL-1β, and IL-6 secretion, using a human THP-1 macrophages model. Therefore, RA content in the basolateral compartment could play an important role in the antioxidant and anti-inflammatory activities found.