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ACC Deaminase from Lysobacter gummosus OH17 Can Promote Root Growth in Oryza sativa Nipponbare Plants

Laborda, Pedro, Ling, Jun, Chen, Xian, Liu, Fengquan
Journal of agricultural and food chemistry 2018 v.66 no.14 pp. 3675-3682
1-aminocyclopropane-1-carboxylate deaminase, 1-aminocyclopropane-1-carboxylic acid, Lysobacter gummosus, Oryza sativa, amination, antifungal properties, bacteria, carboxylic acids, ethylene, ethylene production, genes, rice, root growth
Although Lysobacter species are a remarkable source of natural compounds with antibacterial and antifungal activities, the ability of these bacteria to produce plant growth promoters remains practically unknown. In this work, the ethylene precursor 1-aminocyclopropane-1-carboxylic acid (ACC) has been isolated from the secretions of Lysobacter gummosus OH17, indicating the presence of an ACC deaminase, which was shown to be encoded in the gene peg_1256. The recombinant enzyme could not only deaminate ACC to provide 2-oxobutanoic acid but also catalyzed the amination of the 2-oxobutanoic acid, demonstrating, for the first time, that ACC deaminases can produce ACC. After the treatment of rice Oryza sativa Nipponbare plants with OH17 ACC deaminase, the ethylene production levels were 44% higher in comparison with the control experiments, allowing significant improvements in root, 10%, and stem, 14%, growth.