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Gas Chromatography/Mass Spectrometry-Based Metabolite Profiling of Nutrients and Antinutrients in Eight Lens and Lupinus Seeds (Fabaceae)

Farag, Mohamed A., Khattab, Amira R., Ehrlich, Anja, Kropf, Matthias, Heiss, Andreas G., Wessjohann, Ludger A.
Journal of agricultural and food chemistry 2018 v.66 no.16 pp. 4267-4280
Lens culinaris, Lupinus angustifolius, Lupinus polyphyllus, alkaloids, amino acids, antinutritional factors, breeding, color, cultivars, functional foods, gas chromatography, lentils, mass spectrometry, metabolites, multivariate analysis, nutrients, peptides, phytopharmaceuticals, sterols, sucrose, sugar phosphates
Lens culinaris and several Lupinus species are two legumes regarded as potential protein resources aside from their richness in phytochemicals. Consequently, characterization of their metabolite composition seems warranted to be considered as a sustainable commercial functional food. This study presents a discriminatory holistic approach for metabolite profiling in accessions of four lentil cultivars and four Lupinus species via gas chromatography/mass spectrometry. A total of 107 metabolites were identified, encompassing organic and amino acids, sugars, and sterols, along with antinutrients, viz., alkaloids and sugar phosphates. Among the examined specimens, four nutritionally valuable accessions ought to be prioritized for future breeding to include Lupinus hispanicus, enriched in organic (ca. 11.7%) and amino acids (ca. 5%), and Lupinus angustifolius, rich in sucrose (ca. 40%), along with two dark-colored lentil cultivars ‘verte du Puy’ and ‘Black Beluga’ enriched in peptides. Antinutrient chemicals were observed in Lupinus polyphyllus, owing to its high alkaloid content. Several species-specific markers were also revealed using multivariate data analyses.