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Bacterial Cellulose Relieves Diphenoxylate-Induced Constipation in Rats

Author:
Zhai, Xichuan, Lin, Dehui, Zhao, Yan, Yang, Xingbin
Source:
Journal of agricultural and food chemistry 2018 v.66 no.16 pp. 4106-4117
ISSN:
1520-5118
Subject:
adenosinetriphosphatase, apoptosis, aquaporins, arginine vasopressin, body weight, cellulose, colon, constipation, dietary fiber, enzyme activity, feces, histology, motilin, muscles, myocytes, neurotransmitters, nitric oxide, nitric oxide synthase, rats, secretion, smooth muscle, substance P, vasoactive intestinal peptide, villi
Abstract:
This study was to probe the effects of bacterial cellulose (BC) on diphenoxylate-induced constipation in rats. Administration with BC at 500 mg/kg of body weight in diphenoxylate-induced constipation rats distinctly improved the carmine propulsion rate (83.5 ± 5.2%), shortened the defecating time of the first red feces (249.0 ± 23.3 min), and increased the weight of carmine red feces within 5 h (2.7 ± 1.3 g). The levels of aquaporins (AQP-2, AQP-3, and AQP-4) and inhibitory neurotransmitters (nitric oxide, nitric oxide synthetase, vasoactive intestinal peptide, and arginine vasopressin) in the BC-treated groups reduced by 31.9–40.0% (p < 0.01) and 21.1–67.7% (p < 0.01) compared to those in the constipation group, respectively. However, the secretion of excitability neurotransmitters (substance P and motilin) in the BC-treated groups was increased by 20.0–39.9% (p < 0.01). The activities of ATPases in the colon of constipation rats were significantly weakened by BC administration (p < 0.01). Histological morphology of the colon showed that BC supplementation could effectively increase the length of villus cells and the thickness of colonic mucosa and muscle (p < 0.01). Moreover, BC supplementation could protect colonic smooth muscle cells against apoptosis. All of the findings suggest that BC supplementation effectively relieves constipation in rats and BC would be used as a great promising dietary fiber for alleviating constipation.
Agid:
6042676