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Mimicking a New 2-Phenylethanol Production Pathway from Proteus mirabilis JN458 in Escherichia coli
- Liu, Jinbin, Jiang, Jing, Bai, Yajun, Fan, Tai-ping, Zhao, Ye, Zheng, Xiaohui, Cai, Yujie
- Journal of agricultural and food chemistry 2018 v.66 no.13 pp. 3498-3504
- Escherichia coli, Proteus mirabilis, alcohol dehydrogenase, bacteria, biotransformation, glucose, keto acids, pH, phenylalanine, phenylethyl alcohol
- Bacteria rarely produce natural 2-phenylethanol. We verified a new pathway from Proteus mirabilis JN458 to produce 2-phenylethanol using Escherichia coli to coexpress l-amino acid deaminase, α-keto acid decarboxylase, and alcohol dehydrogenase from P. mirabilis. Based on this pathway, a glucose dehydrogenase coenzyme regeneration system was constructed. The optimal conditions of biotransformation by the recombinant strain E-pAEAKaG were at 40 °C and pH 7.0. Finally, the recombinant strain E-pAEAKaG produced 3.21 ± 0.10 g/L 2-phenylethanol in M9 medium containing 10 g/L l-phenylalanine after a 16 h transformation. Furthermore, when the concentration of l-phenylalanine was 4 g/L (24 mM), the production of 2-phenylethanol reached 2.88 ± 0.18 g/L and displayed a higher conversion rate of 97.38 mol %.