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Effects of High-Pressure Treatment on the Muscle Proteome of Hake by Bottom-Up Proteomics
- Carrera, Mónica, Fidalgo, Liliana G., Saraiva, Jorge A., Aubourg, Santiago P.
- Journal of agricultural and food chemistry 2018 v.66 no.17 pp. 4559-4570
- Merluccius merluccius, biomarkers, creatine kinase, electrophoresis, fructose, fructose-bisphosphate aldolase, glyceraldehyde-3-phosphate dehydrogenase, hake, high pressure treatment, muscles, nucleoside-diphosphate kinase, phosphopyruvate hydratase, proteome, proteomics, triose-phosphate isomerase, tropomyosins
- A bottom-up proteomics approach was applied for the study of the effects of high-pressure (HP) treatment on the muscle proteome of fish. The performance of the approach was established for a previous HP treatment (150–450 MPa for 2 min) on frozen (up to 5 months at −10 °C) European hake (Merluccius merluccius). Concerning possible protein biomarkers of quality changes, a significant degradation after applying a pressure ≥430 MPa could be observed for phosphoglycerate mutase-1, enolase, creatine kinase, fructose bisphosphate aldolase, triosephosphate isomerase, and nucleoside diphosphate kinase; contrary, electrophoretic bands assigned to tropomyosin, glyceraldehyde-3-phosphate dehydrogenase, and beta parvalbumin increased their intensity after applying a pressure ≥430 MPa. This repository of potential protein biomarkers may be very useful for further HP investigations related to fish quality.