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Pilot scale conversion of wheat straw to ethanol via simultaneous saccharification and fermentation
- Saha, Badal C., Nichols, Nancy N., Qureshi, Nasib, Kennedy, Gregory J., Iten, Loren B., Cotta, Michael A.
- Bioresource technology 2015 v.175 pp. 17
- Coniochaeta, Escherichia coli, bacteria, beta-glucosidase, biomass, endo-1,4-beta-glucanase, ethanol, ethanol fermentation, ethanol production, fungi, genetically engineered microorganisms, lignocellulose, pH, saccharification, sugars, sulfuric acid, wheat straw
- The production of ethanol from wheat straw (WS) by dilute acid pretreatment, bioabatement of fermentation inhibitors by a fungal strain, and simultaneous saccharification and fermentation (SSF) of the bio-abated WS to ethanol using an ethanologenic recombinant bacterium was studied at a pilot scale without sterilization. WS (124.2g/L) was pretreated with dilute H2SO4 in two parallel tube reactors at 160°C. The inhibitors were bio-abated by growing the fungus aerobically. The maximum ethanol produced by SSF of the bio-abated WS by the recombinant Escherichia coli FBR5 at pH 6.0 and 35°C was 36.0g/L in 83h with a productivity of 0.43gL−1h−1. This value corresponds to an ethanol yield of 0.29g/g of WS which is 86% of the theoretical ethanol yield from WS. This is the first report on the production of ethanol by the recombinant bacterium from a lignocellulosic biomass at a pilot scale.