U.S. flag

An official website of the United States government

Dot gov

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Https

Secure .gov websites use HTTPS
A lock ( ) or https:// means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.

PubAg

Main content area

Pilot scale conversion of wheat straw to ethanol via simultaneous saccharification and fermentation

Author:
Badal C. Saha, Nancy N. Nichols, Nasib Qureshi, Gregory J. Kennedy, Loren B. Iten, Michael A. Cotta
Source:
Bioresource technology 2015 v.175 pp. 17-22
ISSN:
0960-8524
Subject:
Coniochaeta, Escherichia coli, bacteria, beta-glucosidase, biomass, endo-1,4-beta-glucanase, ethanol, ethanol fermentation, ethanol production, fungi, genetically engineered microorganisms, lignocellulose, pH, saccharification, sugars, sulfuric acid, wheat straw
Abstract:
The production of ethanol from wheat straw (WS) by dilute acid pretreatment, bioabatement of fermentation inhibitors by a fungal strain, and simultaneous saccharification and fermentation (SSF) of the bio-abated WS to ethanol using an ethanologenic recombinant bacterium was studied at a pilot scale without sterilization. WS (124.2g/L) was pretreated with dilute H2SO4 in two parallel tube reactors at 160°C. The inhibitors were bio-abated by growing the fungus aerobically. The maximum ethanol produced by SSF of the bio-abated WS by the recombinant Escherichia coli FBR5 at pH 6.0 and 35°C was 36.0g/L in 83h with a productivity of 0.43gL−1h−1. This value corresponds to an ethanol yield of 0.29g/g of WS which is 86% of the theoretical ethanol yield from WS. This is the first report on the production of ethanol by the recombinant bacterium from a lignocellulosic biomass at a pilot scale.
Agid:
60448
Handle:
10113/60448