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Acute and sub-acute oral toxicity of Brazilian red propolis in rats

da Silva, Rafaela Oliveira, Andrade, Valléria Matos, Bullé Rêgo, Ester Seixas, Azevedo Dória, Grace Anne, Santos Lima, Bruno dos, da Silva, Francilene Amaral, de Souza Araújo, Adriano Antunes, de Albuquerque Júnior, Ricardo Luiz Cavalcanti, Cordeiro Cardoso, Juliana, Zanardo Gomes, Margarete
Journal of ethnopharmacology 2015 v.170 pp. 66-71
adverse effects, bees, chemical composition, guidelines, isoflavones, lethal dose 50, males, medicinal properties, propolis, rats, signs and symptoms (animals and humans), toxicity testing, traditional medicine
Propolis is a bee product widely used in folk medicine due to its numerous pharmacological properties. However, samples from different regions can differ in chemical composition, effectiveness, and side effects. Despite the widespread use of Brazilian red propolis, which is an isoflavone-rich variety, its toxicity has not been carefully studied.To assess the acute and sub-acute toxicity of the hydroethanolic extract of red propolis (HERP) administered orally to rats.HERP for the acute (300mg/kg) and sub-acute (10, 100 and 200mg/kg) toxicity studies was administered orally to rats according to OECD Guidelines 420 and 407, respectively. Clinical signs were identified, and hematological and biochemical analyses were performed. Water and food uptake as well as body and organ weights of animals were recorded.The acute study revealed no lethal effects at 300mg/kg of HERP, but toxic signs were observed, as HERP had an LD50 of more than 300mg/kg, indicating a warning. The most toxic signals in sub-acute studies were observed in males at a dose of 200mg/kg HERP. These results suggest estrogen-like activity, possibly from the isoflavones in HERP.