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Li-Gan-Shi-Liu-Ba-Wei-San improves non-alcoholic fatty liver disease through enhancing lipid oxidation and alleviating oxidation stress

Jiang, Youzhao, Chen, Liu, Wang, Hui, Narisi, Bao, Chen, Bing
Journal of ethnopharmacology 2015 v.176 pp. 499-507
IKappaB kinase, Oriental traditional medicine, animal models, aspartate transaminase, beta oxidation, blood serum, cholesterol, fatty liver, free fatty acids, high fat diet, human cell lines, inducible nitric oxide synthase, lipid peroxidation, liver, liver function, malondialdehyde, oxidative stress, peroxisome proliferator-activated receptors, rats, superoxide dismutase, transcription factor NF-kappa B, triacylglycerols
Mongolian medicine is an important constituent of traditional Chinese medicine. Its representative prescription, Li-Gan-Shi-Liu-Ba-Wei-San (LGSLBWS), is widely used for long-term treatment of chronic liver disease and nonalcoholic fatty liver disease (NAFLD).This study explored the effects and mechanism of LGSLBWS on NAFLD.NAFLD rat model was established with high-fat diet. The effects of LGSLBWS on lipid metabolism, liver function, and hepatic morphology were observed in NAFLD rats. Superoxide dismutase (SOD) and malondialdehyde (MDA) contents in the liver, as well as the expression levels of peroxisome proliferator-activated receptor (PPAR)α, PPARβ, inhibitor of nuclear factor κB α(IκBα), and inducible nitric oxide synthase (iNOS) were all detected. Finally, the effects of LGSLBWS on fatty acid oxidation, PPARα, PPARβ, IκBα, and iNOS were determined in HepG2 cells.LGSLBWS significantly reduced the fat deposition in the liver and the serum aspartate aminotransferase levels in NAFLD rats. Serum triglyceride and free fatty acid levels were reduced by LGSLBWS. Total cholesterol and triglyceride contents in the liver were also downregulated. SOD and MDA levels were increased and decreased by LGSLBWS, respectively. LGSLBWS can significantly promote fatty acid oxidation of HepG2 cells. Upregulation of PPARα, PPARβ, and IκBα and downregulation of iNOS by LGSLBWS were both observed in the NAFLD model and HepG2 cells.LGSLBWS can significantly improve NAFLD by enhancing fatty acid oxidation and alleviating oxidative stress.