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Fluorescence Polarization Immunoassay for Alternaria Mycotoxin Tenuazonic Acid Detection and Molecular Modeling Studies of Antibody Recognition
- Wang, Feng, Cai, Jun, Eremin, Sergei A., Xiao, Zhi-Li, Shen, Yu-Dong, Tian, Yuan-Xin, Xu, Zhen-Lin, Yang, Jin-Yi, Lei, Hong-Tao, Sun, Yuan-Ming, Wang, Hong
- Food analytical methods 2018 v.11 no.9 pp. 2455-2462
- Alternaria, analytical methods, antibodies, energy, fluorescein, fluorescence, haptens, immunoassays, inhibitory concentration 50, isomers, isothiocyanates, molecular models, mycotoxins, rapid methods, tenuazonic acid, tracer techniques
- A simple and rapid method of fluorescence polarization immunoassay (FPIA) for detection of Alternaria mycotoxin tenuazonic acid (TeA) was described. In this study, the tracers TeAH-FITC and TeAH-DTAF were synthesized from hapten 5-(sec-butyl)-3-(1-hydrazonoethyl)-4-hydroxy-1H-pyrrol-2(5H)-one (TeAH) and different fluoresceins isothiocyanate isomer I (FITC) or 5-([4,6-dichlorotriazin-2-yl]amino)fluorescein hydrochloride (DTAF), respectively. The established FPIA showed an IC₅₀ value of 3.0 μg/mL with a detection limit of 0.13 μg/mL for TeAH based on the tracer of TeAH-FITC, and with the same properties of 7.3 and 0.93 μg/mL based on TeAH-DTAF. The former gave better assay sensitivity than the latter, that the 2,4-dichloro-s-triazine group of TeAH-DTAF may affect the specific recognition of tracer and antibody. Models of the lowest energy conformation and the molecular electrostatic potential isosurface studies revealed that the –C=N–N– and –CHCH₃(CH₂CH₃) groups were the characteristic chemical groups in anti-TeAH antibody-analyte recognition via molecular modeling, which could create a theoretical guide for hapten design of small molecules and provide the traditional theory with empirical proof.