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Camelina sativa Defatted Seed Meal Contains Both Alkyl Sulfinyl Glucosinolates and Quercetin That Synergize Bioactivity

Author:
Nilanjan Das, Mark A. Berhow, Donato Angelino, Elizabeth H. Jeffery
Source:
Journal of agricultural and food chemistry 2014 v.62 no.33 pp. 8385-8391
ISSN:
0021-8561
Subject:
broccoli, NAD(P)H dehydrogenase (quinone), metabolic detoxification, glucosinolates, oilmeals, cytochrome P-450, oilseed crops, hydrolysis, Camelina sativa, quercetin, anticarcinogenic activity
Abstract:
Camelina sativa L. Crantz is under development as a novel oil-seed crop, yet bioefficacy of camelina phytochemicals is unknown. Defatted camelina seed meal contains two major aliphatic glucosinolates (GSL), glucoarabin (9-(methylsulfinyl)nonylglucosinolate; GSL 9) and glucocamelinin (10-(methylsulfinyl)decylglucosinolate; GSL 10), with traces of a third, 11(methylsulfinyl)undecylglucosinolate and several flavonoids, mostly quercetin glycosides. In Hepa1c1c7 cells, hydrolyzed GSL (hGSL) 9 and 10 upregulated the phase II detoxification enzyme quinone reductase (NQO1), with no effect on cytochrome P450 (CYP) 1A1 activity. Isobologram graphs revealed synergy of NQO1 induction for a combination of hGSL9 and quercetin. These findings suggest that defatted camelina seed meal should be evaluated for anticancer activity, similar to broccoli and other Brassicaceae family members. Interestingly, synergy of NQO1 induction was also seen for physiologically relevant doses of sulforaphane (SF) and quercetin, two key bioactives present in broccoli. This suggests that SF within broccoli may be more potent than purified SF.
Agid:
60477
Handle:
10113/60477