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Frequency of virulence factors in Helicobacter pylori-infected patients with gastritis
- Salimzadeh, Loghman, Bagheri, Nader, Zamanzad, Behnam, Azadegan-Dehkordi, Fatemeh, Rahimian, Ghorbanali, Hashemzadeh-Chaleshtori, Morteza, Rafieian-Kopaei, Mahmoud, Sanei, Mohammad Hossein, Shirzad, Hedayatollah
- Microbial pathogenesis 2015 v.80 pp. 67-72
- Helicobacter pylori, biopsy, cytotoxins, gastritis, genes, genotype, histology, histopathology, inflammation, neutrophils, patients, polymerase chain reaction, virulence
- The outcome of Helicobacter pylori infection has been related to speciﬁc virulence-associated bacterial genotypes. The vacuolating cytotoxin (vacA), cagA gene, oipA and babA2 gene are important virulence factor involving gastric diseases. The objective of this study was to assess the relationship between virulence factors of H. pylori and histopathological ﬁndings.Gastroduodenoscopy was performed in 436 dyspeptic patients. Antrum biopsy was obtained for detection of H. pylori, virulence factors and for histopathological assessment. The polymerase chain reaction was used to detect virulence factors of H. pylori using speciﬁc primers.vacA genotypes in patients infected with H. pylori were associated with cagA, iceA1 and iceA2. In the patients with H. pylori infection there was a signiﬁcant relationship between cagA positivity and neutrophil activity (P = 0.004) and chronic inflammation (P = 0.013) and with H. pylori density (P = 0.034). Neutrophil inﬁltration was found to be more severe in the s1 group than in the s2 group (P = 0.042). Also was a signiﬁcant relationship between oipA positivity and neutrophil activity (P = 0.004) and with H. pylori density (P = 0.018). No signiﬁcant relationships were observed between other vacA genotypes and histopathological parameters.H. pylori strains showing cagA, vacA s1 and oipA positivity are associated with more severe gastritis in some histological features but virulence factors of H. pylori do not appear to determine the overall pattern of gastritis.