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Direct E-DNA sensor of Mycobacterium tuberculosis mutant strain based on new nanocomposite transducer (Fc-ac-OMPA/MWCNTs)

Bizid, Salma, Blili, Saber, Mlika, Rym, Haj Said, Ayoub, Korri-Youssoufi, Hafsa
Talanta 2018 v.184 pp. 475-483
DNA, DNA probes, Mycobacterium tuberculosis, bacteria, biosensors, drugs, electrochemistry, hepatitis C, moieties, monitoring, mutants, nanocomposites, nucleic acid hybridization, rifampicin, single nucleotide polymorphism
Direct DNA sensor based on new nanocomposite materials (Fc-ac-OMPA/MWCNTs) has been investigated. This nanocomposite was formed by combining the redox oligomer “oligo-methoxy-phenyl-acetonitrile” (Fc-ac-OMPA) and the MWCNTs via a simple π-π stacking interaction in the aim to ameliorate the biosensor performance. The redox indicator and the functional groups of the redox oligomer have been used for monitoring the electrochemical behavior and the flexibility for direct covalent attachment of Hepatitis C DNA probe. This nanocomposite shows high performance of DNA hybridization with a detection limit of 0.08 fmol L−1. Moreover, the biosensor was applied for the detection of pathogenic bacterium such as DNA from Mycobacterium tuberculosis strand. Developed biosensor has been able to detect a single nucleotide polymorphism (SNP)T (TCG/ TTG) which confers resistance of M. tuberculosis to rifampicin drug.