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Technical Note: Transfer of caprine blastocysts vitrified by the open pulled straw (OPS) or the solid surface procedure and warmed in sucrose-free medium

Author:
Garza, D., Camacho, M., Gauly, M., Holtz, W.
Source:
Small ruminant research 2018 v.165 pp. 111-114
ISSN:
0921-4488
Subject:
blastocyst, goats, kidding rate, liquids, nitrogen, vitrification
Abstract:
The present paper outlines ways of non-surgical collection and semi-laparoscopic transfer of caprine embryos. Two different ways of embryo cryo-preservation by way of vitrification are described; the open pulled straw (OPS) procedure, known to be well suited and the solid surface procedure called for in situations where contact between embryos and non-sterile liquid nitrogen is to be avoided. Based on 13 transfers of OPS-vitrified and 9 transfers of solid surface-vitrified blastocysts (2 blastocysts/recipient) it was shown that either procedure is applicable (54% vs. 56% pregnancy- and 39% vs. 44% kidding rate). Furthermore the experiment showed that warming of vitrified embryos may be accomplished by one-step procedure (88% transferable post-warming embryos), opening up the possibility to transfer vitrified embryos under field conditions.
Agid:
6054021