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Integrated antibacterial and antifouling surfaces via cross-linking chitosan-g-eugenol/zwitterionic copolymer on electrospun membranes B Biointerfaces

Li, Zhenguang, Hu, Wenhong, Zhao, Yunhui, Ren, Lixia, Yuan, Xiaoyan
Colloids and surfaces 2018 v.169 pp. 151-159
Escherichia coli, Staphylococcus aureus, adhesion, adsorption, antibacterial properties, anticoagulant activity, antifouling activities, bacterial adhesion, biocompatibility, biocompatible materials, blood platelets, cell culture, composite polymers, crosslinking, cytotoxicity, fibroblasts, growth retardation, hemolysis, urethane, viability, zwitterions
Integrated antibacterial and antifouling surfaces in favor of avoiding implant-related infections are necessarily required for biomaterials when they contact with the body fluid. In this work, an antibacterial and antifouling membrane was developed via cross-linking chitosan-g-eugenol and the zwitterionic copolymer poly(sulfobetaine methylacrylate-co-2-aminoethyl methacrylate) on the electrospun polycarbonate urethane substrate using genipin as a cross-linker. Antibacterial assays demonstrated that the prepared membranes had efficient antibacterial activity with 92.8 ± 2.5% and 95.2 ± 1.3% growth inhibition rates against Escherichia coli and Staphylococcus aureus, respectively. The investigations on antifouling activity and hemocompatibility of the membranes showed significant resistances to bacterial attachment, non-specific protein adsorption and platelet adhesion, and presented lower hemolytic activity and good anticoagulant activity as well. Moreover, cell culture assays indicated that the prepared membranes exerted no obvious cytotoxicity with more than 80% of relative L929 fibroblast viability. Therefore, the membranes with integrated antibacterial and antifouling properties could be potentially applied in promising indwelling devices.