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Hydrothermal conversion of Magnolia liliiflora into nitrogen-doped carbon dots as an effective turn-off fluorescence sensing, multi-colour cell imaging and fluorescent ink B Biointerfaces

Author:
Atchudan, Raji, Edison, Thomas Nesakumar Jebakumar Immanuel, Aseer, Kanikkai Raja, Perumal, Suguna, Lee, Yong Rok
Source:
Colloids and surfaces 2018 v.169 pp. 321-328
ISSN:
0927-7765
Subject:
Magnolia liliiflora, carbon quantum dots, cytoplasm, detection limit, dispersibility, flowers, fluorescence, hepatocytes, hot water treatment, image analysis, industrial effluents, ions, iron, moieties, nitrogen, rats, zeta potential
Abstract:
The present work illustrates the potential uses of nitrogen-doped multi-fluorescent carbon dots (N-CDs) for Fe³⁺ sensing, cellular multi-colour imaging, and fluorescent ink. N-CDs were synthesized using Magnolia liliiflora flower by the simple hydrothermal method. The resulted N-CDs was found to be nearly spherical in shape with the size of about 4 ± 1 nm and showed competitive quantum yield around 11%. The synthesized N-CDs with uniform size distribution and high content of nitrogen and oxygen-bearing functional groups exhibit excellent dispersibility in aqueous media. The N-CDs were able to detect a high concentration of Fe³⁺ ions (1–1000 μM) with a limit of detection is about 1.2 μM by forming N-CDs-Fe³⁺ complex due to the functional groups such as nitrogen, carbonyl and carboxyl on the surface of N-CDs. Thus they could be used to remove pollutants from industrial wastewater. The electronic charge on the surface of the N-CDs and N-CDs-Fe³⁺ complex (zeta potential) is around −36 and 18 mV, respectively. In addition, these N-CDs show excitation-dependent fluorescence that was utilized for multi-colour in vitro cellular imaging in rat liver cells (Clone 9 hepatocytes). The N-CDs are rapidly uptake in the cell cytoplasm and showed high cytocompatibility on cellular morphology. Moreover, as the N-CDs possess strong fluorescence and anti-coagulation they could be utilized in fluorescent ink pens.
Agid:
6054198