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Antioxidant and antimicrobial properties of phenolic rich fraction of Seabuckthorn (Hippophae rhamnoides L.) leaves in vitro

Yogendra Kumar, M.S., Tirpude, R.J., Maheshwari, D.T., Bansal, Anju, Misra, Ksipra
Food chemistry 2013 v.141 no.4 pp. 3443-3450
gallic acid, myricetin, Staphylococcus aureus, antibacterial properties, nitric oxide, Hippophae rhamnoides, Streptococcus pneumoniae, quercetin, reversed-phase high performance liquid chromatography, rutin, antioxidant activity, Shigella dysenteriae, Salmonella Typhi, leaves, Escherichia coli
Phenolic rich fraction (PRF) from Seabuckthorn leaves was prepared by sequential fractionation. Total phenolic content of PRF estimated as gallic acid equivalent was found to be 319.33±7.02mg/g of PRF. Its major constituents gallic acid, rutin, quercetin-3-galactoside, quercetin-3-glucoside, myricetin, quercetin, kaempferol and isorhamnetin, were found in the range of 1.551–196.89mg/g of PRF as determined by RP-HPLC. Antioxidant activity of PRF evaluated using 2,2-diphenyl-2-picrylhydrazyl, superoxide and nitric oxide scavenging assays. Reducing power of PRF increased with increasing amount of PRF; the equation of reducing power (y) and amount of PRF (x) was y=8.004x (r2=0.99), indicating that reducing ability correlated well with amount of PRF.Antibacterial activity of PRF, tested against certain medically important bacterial species showed growth inhibiting effect against Escherichia coli, Salmonella typhi, Shigella dysenteriae, Streptococcus pneumoniae and Staphylococcus aureus. In conclusion, PRF has potent antioxidant and broad spectrum antibacterial properties.