Jump to Main Content
A Muscle-Specific Enhancer RNA Mediates Cohesin Recruitment and Regulates Transcription In trans
- Tsai, Pei-Fang, Dell’Orso, Stefania, Rodriguez, Joseph, Vivanco, Karinna O., Ko, Kyung-Dae, Jiang, Kan, Juan, Aster H., Sarshad, Aishe A., Vian, Laura, Tran, Michelle, Wangsa, Darawalee, Wang, A. Hongjun, Perovanovic, Jelena, Anastasakis, Dimitrios, Ralston, Evelyn, Ried, Thomas, Sun, Hong-Wei, Hafner, Markus, Larson, Daniel R., Sartorelli, Vittorio
- Molecular cell 2018 v.71 no.1 pp. 129-141.e8
- RNA, cell differentiation, chromatin, gene expression, genes, loci, myogenin, transcription (genetics)
- The enhancer regions of the myogenic master regulator MyoD give rise to at least two enhancer RNAs. Core enhancer eRNA (CᴱeRNA) regulates transcription of the adjacent MyoD gene, whereas ᴰᴿᴿeRNA affects expression of Myogenin in trans. We found that ᴰᴿᴿeRNA is recruited at the Myogenin locus, where it colocalizes with Myogenin nascent transcripts. ᴰᴿᴿeRNA associates with the cohesin complex, and this association correlates with its transactivating properties. Despite being expressed in undifferentiated cells, cohesin is not loaded on Myogenin until the cells start expressing ᴰᴿᴿeRNA, which is then required for cohesin chromatin recruitment and maintenance. Functionally, depletion of either cohesin or ᴰᴿᴿeRNA reduces chromatin accessibility, prevents Myogenin activation, and hinders muscle cell differentiation. Thus, ᴰᴿᴿeRNA ensures spatially appropriate cohesin loading in trans to regulate gene expression.