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Wolbachia infection differs among divergent mitrochondrial haplotypes of Bactericera cockerelli (Hemiptera: Triozidae)

W. Rodney Cooper, Kylie D. Swisher, Stephen F. Garczynski, Tariq Mustafa, Joseph E. Munyaneza, David R. Horton
Annals of the Entomological Society of America 2015 v.108 no.2 pp. 137-145
Wolbachia, haplotypes, mitochondrial DNA, Bactericera cockerelli, geographical variation, males, polymerase chain reaction, endosymbionts, female fertility, cytochrome-c oxidase, Triozidae, eggs, females, bacteria, North America
Four mitochondrial (cytrochrome oxidase I) haplotypes of the potato psyllid, Bactericera cockerelli (Sˇulc) (Hemiptera: Triozidae), have been identified in North America: western, central, northwestern, and southwestern. A recent study found that females of the northwestern haplotype mated by males of the western or central haplotypes failed to produce viable eggs. Our goal was to determine whether these patterns in reproductive incompatibility are associated with differences among haplotypes in the presence of cytoplasmic incompatibility-inducing bacteria, Wolbachia or Cardinium. Polymerase chain reaction (PCR) revealed that psyllids of the western and central haplotypes were both simultaneously infected with two strains ofWolbachia, butWolbachia was not detected in psyllids of the northwestern haplotype. PCR using archived DNA from field-collected psyllids confirmed patterns in Wolbachia infection among the western, central, and northwestern haplotypes, and also indicated thatWolbachia was not detectable in psyllids of the southwestern haplotype, which were not available for the mating studies. Cardinium was not detected in psyllids regardless of haplotype. These results provide evidence that differences in Wolbachia infection may be the cause of cytoplasmic incompatibilities among sympatric yet biologically distinct populations of B. cockerelli that have highly divergent mitochondrial haplotypes. This knowledge will improve the interpretation of studies to assess interactions and biological differences among B. cockerelli haplotypes.