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Characterization of a Whole-Cell Biotransformation Using a Constitutive Lysine Decarboxylase from Escherichia coli for the High-Level Production of Cadaverine from Industrial Grade L-Lysine

Author:
Shin, Jihyun, Joo, Jeong Chan, Lee, Eunji, Hyun, Sung Min, Kim, Hyun Joong, Park, Si Jae, Yang, Yung-Hun, Park, Kyungmoon
Source:
Applied biochemistry and biotechnology 2018 v.185 no.4 pp. 909-924
ISSN:
0273-2289
Subject:
Escherichia coli, biocatalysts, biotransformation, cadaverine, lysine, lysine decarboxylase, pH
Abstract:
Cadaverine is used for the synthesis of the novel bio-polyamides 54, 56, and 510. Here, we examine the feasibility of using a lysine decarboxylase (LdcC) from Escherichia coli for high-level production of cadaverine. After sequential optimization of whole-cell biotransformation conditions, recombinant E. coli-overexpressing LdcC (EcLdcC) could produce 1.0 M cadaverine from 1.2 M crude L-lysine solution after 9 h. EcLdcC retained a higher cadaverine yield after being reused 10 times at acidic and alkaline pH values than that of a recombinant E. coli strain overexpressing an inducible lysine decarboxylase (CadA), a conventional cadaverine producer (90 vs. 51% at pH 6 and 55 vs. 15% at pH 8). This study reveals that EcLdcC is a promising whole-cell biocatalyst for the bio-based production of cadaverine from industrial grade L-lysine in comparison to EcCadA.
Agid:
6062077