U.S. flag

An official website of the United States government

Dot gov

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Https

Secure .gov websites use HTTPS
A lock ( ) or https:// means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.

PubAg

Main content area

Have we found an Optimal Insertion Site in a Newcastle Disease Virus Vector to Express a Foreign Gene for Vaccine and Gene Therapy Purposes?

Author:
Qingzhong Yu
Source:
British journal of virology 2015 v.2 no.2 pp. 15-18
ISSN:
2055-6128
Subject:
gene therapy, green fluorescent protein, quantitative analysis, fluorescence, vaccines, gene expression, Avian orthoavulavirus 1, virology, genes, genetic vectors
Abstract:
Different approaches have been used to investigate the most suitable insertion site for optimal expression of a foreign gene without compromising the replication of the Newcastle disease virus. Recently, inserting a green fluorescent protein (GFP) gene upstream from the GE sequences of the viral genes and subsequent quantitative measurements of the GFP fluorescence intensity, we have concluded that the P and M junction region is the optimal insertion site for a high level of foreign gene expression by a NDV vector.
Agid:
60769
Handle:
10113/60769