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Have we found an Optimal Insertion Site in a Newcastle Disease Virus Vector to Express a Foreign Gene for Vaccine and Gene Therapy Purposes?

Yu, Qingzhong
British journal of virology 2015 v.2 no.2 pp. 15-18
Newcastle disease virus, fluorescence, gene expression, gene therapy, genes, genetic vectors, green fluorescent protein, quantitative analysis, vaccines, virology
Different approaches have been used to investigate the most suitable insertion site for optimal expression of a foreign gene without compromising the replication of the Newcastle disease virus. Recently, inserting a green fluorescent protein (GFP) gene upstream from the GE sequences of the viral genes and subsequent quantitative measurements of the GFP fluorescence intensity, we have concluded that the P and M junction region is the optimal insertion site for a high level of foreign gene expression by a NDV vector.