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Fabrication of a Double-Cross-Linked Interpenetrating Polymeric Network (IPN) Hydrogel Surface Modified with Polydopamine to Modulate the Osteogenic Differentiation of Adipose-Derived Stem Cells

Author:
Pacelli, Settimio, Rampetsreiter, Kyle, Modaresi, Saman, Subham, Siddharth, Chakravarti, Aparna R., Lohfeld, Stefan, Detamore, Michael S., Paul, Arghya
Source:
ACS applied materials & interfaces 2018 v.10 no.30 pp. 24955-24962
ISSN:
1944-8252
Subject:
adsorption, alginates, bone formation, coatings, dexamethasone, gelatin, hydrogels, stem cells
Abstract:
Hydrogel surface properties can be modified to form bioactive interfaces to modulate the osteogenic differentiation of stem cells. In this work, a hydrogel made of gelatin methacrylamide (GelMA) and alginate was designed and tested as a scaffold to control stem-cell osteogenic differentiation. The hydrogel’s surface was treated with polydopamine (pDA) to create an adhesive layer for the adsorption of the osteoinductive drug dexamethasone (Dex). The presence of the pDA coating enhanced Dex adsorption and retention over 21 days. This effect resulted in a delay in the osteogenic differentiation of hASCs cultured on the hydrogel treated with a pDA layer.
Agid:
6079033