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Changes in expression of proteins involved in alleviation of Fe-deficiency by sulfur nutrition in Brassica napus L.

Muneer, Sowbiya, Lee, Bok Rye, Bae, Dong Won, Kim, Tae Hwan
Acta physiologiae plantarum 2013 v.35 no.10 pp. 3037-3045
Brassica napus var. napus, RNA, chloroplasts, chlorosis, hydrogen peroxide, iron, leaves, nutrition, protein synthesis, proteins, sulfur
In order to characterize the significance of sulfur (S) nutrition in protein expression under iron (Fe)-deficient conditions, gel-based proteomic analysis was performed with the leaves of Brassica napus exposed to S and Fe combined treatments: sufficient in S and Fe (+S/+Fe, control), sufficient S but Fe deprived (+S/−Fe), deprived S but sufficient Fe (−S/+Fe), and deprived S and Fe (−S/−Fe). The resulting data showed that 15 proteins were down-regulated due to production of oxidative damage as indicated by H₂O₂and O₂⁻¹localizations and due to leaf chlorosis in leaves in S-deprived leaves either in presence (−S/+Fe) or absence of Fe (−S/−Fe), whereas these down-regulated proteins were well expressed in the presence of S (+S/−Fe) compared to control (+S/+Fe). In addition, two proteins were up-regulated under S-deprived condition in presence (−S/+Fe) and absence of (−S/−Fe) Fe. The functional classification of these identified proteins was estimated that 40 % of the proteins belong to chloroplast precursor, and rest of the proteins belongs to hypothetical proteins, RNA binding, secondary metabolism and unknown proteins. On the other hand, five protein spots from S deprived (−S/+Fe) and ten spots from Fe deprived (−S/−Fe) conditions were absent, whereas they were well expressed in presence of S (+S/−Fe) compared to control plants (+S/+Fe). These results suggest that sulfur nutrition plays an important role in alleviating protein damage in Fe-deficient plants and adaptation to Fe-deficiency in oilseed rape.