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Anti-inflammatory and antioxidant activities of aqueous extract of Cecropia glaziovii leaves

Müller, Simony Davet, Florentino, Drielly, Ortmann, Caroline Flach, Martins, Fernanda Amélia, Danielski, Lucineia Gainski, Michels, Monique, de Souza Constantino, Larissa, Petronilho, Fabricia, Reginatto, Flavio Henrique
Journal of ethnopharmacology 2016 v.185 pp. 255-262
Cecropia, animal models, antioxidant activity, cell movement, chlorogenic acid, dexamethasone, diabetes, free radicals, high performance liquid chromatography, hydrogen peroxide, interleukin-1beta, interleukin-6, lactate dehydrogenase, leaf extracts, leaves, lipids, myeloperoxidase, nitrates, nitrites, pleurisy, protein content, proteins, thiobarbituric acid-reactive substances, traditional medicine, tumor necrosis factor-alpha, Brazil
Cecropia glaziovii Sneth leaves extract is widely used as a traditional folk medicine in Brazil, especially for the treatment of diabetes, and as an antihypertensive and antiinflammatory agent.To investigate the anti-inflammatory and antioxidant properties of crude aqueous extract (CAE) of C. glaziovii leaves.The in vivo anti-inflammatory and antioxidant effect of the CAE (10-300mg/kg, intragastrically) was investigated in the animal model of pleurisy. The cell migration, proinflammatory cytokines (TNF-α, IL-1β and IL-6), nitrite/nitrate concentration, myeloperoxidase (MPO) activity, oxidative damage in lipids and proteins, lactate dehydrogenase (LDH) activity and total protein content were also analyzed. Furthermore, the in vitro antioxidant activity of CAE was evaluated by the inhibition of formation of thiobarbituric acid reactive substances (TBARS), induced by free radical generators (H2O2, FeSO4 and AAPH) on a lipid-rich substrate. Hence, the chemical characterizarion of CAE by HPLC was therefore performed.The results showed that the inflammatory process caused by the administration of carragenin (Cg) into the pleural cavity resulted in a substantial increase in inflammatory parameters and oxidative damage. These levels seems to be reversed after CAE treatment in animals with similar results to Dexamethasone (Dex) treatment. Further, the CAE was effective in reducing proinflammatory cytokines, cell infiltrate, MPO activity, nitrite/nitrate concentration, LDH activity, and total protein levels with concomitant attenuation of all parameters associated with oxidative damage induced by Cg. Finally, the CAE presented in vitro antioxidant activity induced by free radical generators at all the concentrations investigated. HPLC analysis confirmed the presence of chlorogenic acid and C-glycosylflavonoids (isoorientin and isovitexin) as the major compounds of the CAE.CAE of C. glaziovii exerts significant antiinflammatory and antioxidant activities and this effect can be attributed, at least in part, to the presence of chlorogenic acid and the C-glycosylflavonoids.