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Biosynthesis of palladium nanoparticles by using Moringa oleifera flower extract and their catalytic and biological properties

Anand, K., Tiloke, C., Phulukdaree, A., Ranjan, B., Chuturgoon, A., Singh, S., Gengan, R.M.
Journal of photochemistry and photobiology 2016 v.165 pp. 87-95
Artemia, Bacillus cereus, Candida albicans, Candida utilis, Enterococcus faecalis, Escherichia coli, Fourier transform infrared spectroscopy, Moringa oleifera, Staphylococcus aureus, acetates, agroforestry, antibacterial properties, aqueous solutions, biomass, biosynthesis, biphenyl, catalysts, cell lines, cell viability, chemical composition, chemical reactions, color, cytotoxicity, death, effluents, energy-dispersive X-ray analysis, flowers, gas chromatography-mass spectrometry, humans, lung neoplasms, lymphocytes, methylene blue, nanoparticles, neoplasm cells, p-nitrophenol, palladium, scanning electron microscopy, toxicity testing, transmission electron microscopy, ultraviolet-visible spectroscopy, viability assays
The biosynthesis of nanostructured biopalladium nanoparticles (PdNPs) from an aqueous solution of crystalline palladium acetate is reported. For the synthesised PdNPs in solution, an agroforest biomass waste petal of Moringa oleifera derived bis-phthalate was used as natural reducing and biocapping agents. Continuous absorption in the UV region and subsequent brown colour change confirmed the formation of PdNPs. A strong surface plasmon peak for PdNPs occurred at 460nm. PdNPs were characterized by SEM with EDX, FTIR, TEM and DLS. The chemical composition of the aqueous extract was determined by GC–MS coupled with FTIR and 1NMR. The catalytic degradation effect by PdNPs on industrial organic toxic effluents p-nitrophenol (PNP) and methylene blue dye was monitored by UV Spectroscopy. On the other hand PdNPs catalysed the base mediated suzuki coupling reaction for biphenyl synthesis, in water. Moreover, PdNPs were found to be reusable catalysts. Toxicity studies of PdNPs showed that the death of brine shrimp to be <50%. Therefore, PdNPs displayed potential for further anticancer studies via tumour cell lines. The in vitro cytotoxicity evaluation of the extract capped nanoparticles was carried out using human lung carcinoma cells (A549) and peripheral lymphocytes normal cells by MTT cell viability assay. Also, PdNPs showed antibacterial activity against Enterococcus faecalis among the different tested strains, including Bacillus cereus, Staphylococcus aureus, Esherichia coli and Candida albicans, Candida utilis.