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Estimating the Genetic Relationship in Various Warm Season Turfgrass Genotypes Using RAPD and ISSR Markers

Agnihotri, Roshni, Chawla, Shiv Lal, Srivashtav, Vishal
National Academy science letters 2018 v.41 no.4 pp. 197-201
Axonopus compressus, Cynodon dactylon, Stenotaphrum secundatum, Zoysia matrella, Zoysia tenuifolia, cluster analysis, genetic relationships, genetic variation, genotype, microsatellite repeats, random amplified polymorphic DNA technique, turf grasses, warm season
The genetic diversity of 13 warm season turfgrass genotypes were studied in which 7 genotypes is of Cynodon dactylon and one each of C. dactylon × C. transvaalensis, Zoysia matrella, Zoysia tenuifolia, Stenotaphrum secundatum, Axonopus compressus and Eremochola ophiuroides were assessed using PCR based Random Amplified Polymorphic DNA (RAPD) and Inter Simple Sequence Repeat (ISSR) markers. Out of 150 primers of RAPD and 18 primers of ISSR screened, six each of RAPD and ISSR primers, were used to generate polymorphism for all genotypes. The dendrogram obtained from UPGMA cluster analysis of Jaccard’s similarity values ranged from 0.029 to 0.395 for RAPD and from 0.085 to 0.750 for ISSR primers. Based on above markers the genotypes were grouped into six distinct clusters.