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Analysis of the biological and molecular variability of the Polish isolates of Tomato black ring virus (TBRV)
- Rymelska, N., Borodynko, N., Pospieszny, H., Hasiów-Jaroszewska, B.
- Virus genes 2013 v.47 no.2 pp. 338-346
- RNA, Tomato black ring virus, genetic variation, genome, host range, pathogens, phylogeny, restriction fragment length polymorphism
- Tomato black ring virus (TBRV) is an important pathogen infecting many plant species worldwide. The biological and molecular variability of the Polish isolates of TBRV was analyzed. The analysis was performed based on the symptoms induced by various isolates on test plant species as well as on phylogenetic relationships between isolates. Isolates differed in their host range and symptomatology. In addition, genetic variation among isolates was characterized by restriction fragment length polymorphism analysis and confirmed by sequencing. The phylogenetic analysis revealed that the Polish isolates differ from each other and do not form a monophyletic cluster. Finally, we identified and analyzed sequences of defective RNA forms arising from the TBRV genome.