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A probe-based quantitative PCR assay for detecting Tetracapsuloides bryosalmonae in fish tissue and environmental DNA water samples

Author:
Hutchins, Patrick R., Sepulveda, Adam J., Martin, Renee M., Hopper, Lacey R.
Source:
Conservation genetics resources 2018 v.10 no.3 pp. 317-319
ISSN:
1877-7252
Subject:
DNA, Salmonidae, calibration, detection limit, diagnostic techniques, fish, kidney diseases, kidneys, quantitative polymerase chain reaction
Abstract:
A probe-based quantitative real-time PCR assay was developed to detect Tetracapsuloides bryosalmonae, which causes proliferative kidney disease in salmonid fish, in kidney tissue and environmental DNA (eDNA) water samples. The limits of detection and quantification were 7 and 100 DNA copies for calibration standards and T. bryosalmonae was reliably detected down to 100 copies in tissue and eDNA samples. The assay presented here is a highly sensitive and quantitative tool for detecting T. bryosalmonae with potential applications for tissue diagnostics and environmental detection.
Agid:
6089100