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Characterization of the complete chloroplast genome sequence of Littledalea racemosa Keng (Poaceae: Bromeae)

Liu, Yuping, Su, Xu, Lv, Ting, Liu, Tao, Chen, Kelong
Conservation genetics resources 2018 v.10 no.3 pp. 343-346
Poaceae, chloroplast genome, chloroplasts, genes, grasses, nucleotide sequences, phylogeny, ribosomal RNA, transfer RNA, China
Littledalea racemosa is an alpine perennial species in the grass (Poaceae) tribe Bromeae that is endemic to the Qinghai–Tibet Plateau and adjacent regions. In the present study, we sequenced the complete chloroplast (cp) genome of L. racemosa on the Illumina MiSeq platform. This represents the first complete cp sequence for the Bromeae. The total genome size was 135,330 bp in length with 38.5% GC content. It contains a pair of inverted repeat regions (21,429 bp) separated by a small single copy region (12,486 bp) and a large single copy region (79,986 bp). Within the newly sequenced cp genome, we annotated a total of 142 genes, which included 84 protein-coding genes, 38 tRNAs genes, and 8 rRNAs genes. The most of genes occur within the single copy regions. We used the cp genome of L. racemosa and 37 other chloroplast genomes to perform a phylogenetic analysis, which indicated that L. racemosa is closely related species in tribe Triticeae of subfamily Pooideae.