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Protective Effects of Quercetin Against Cadmium Chloride-Induced Oxidative Injury in Goat Sperm and Zygotes

Author:
Mao, Tingchao, Han, Chengquan, Wei, Biao, Zhao, Lu, Zhang, Qing, Deng, Ruizhi, Liu, Jun, Luo, Yan, Zhang, Yong
Source:
Biological trace element research 2018 v.185 no.2 pp. 344-355
ISSN:
0163-4984
Subject:
antioxidant activity, cadmium, catalase, embryogenesis, gene expression, glutathione peroxidase, goats, malondialdehyde, mitochondria, oxidative stress, peroxidation, protective effect, quercetin, reactive oxygen species, sperm quality, superoxide dismutase, survival rate, zygote
Abstract:
Quercetin, a plant-derived flavonoid, is frequently used as an antioxidant for efficient anti-oxidative capacity. However, whether quercetin has protective effects on goat sperm and preimplantation embryos against Cd²⁺-induced oxidative injury is still unclear. So, we researched the influence of quercetin on goat sperm and zygotes respectively under the oxidative stress induced by Cd²⁺. In our study, quercetin decreased the malonaldehyde (MDA) and reactive oxygen species (ROS) levels caused by Cd²⁺ in goat sperm (p < 0.05), which facilitated sperm characteristics including motility, survival rates, membrane integrity, and mitochondria activity during storage in vitro and subsequent embryo development (p < 0.05). Moreover, in goat zygotes, quercetin decreased peroxidation products including ROS, MDA, and carbonyl through preserving or maintaining mitochondrial function, gene expression, and anti-oxidative products such as glutathione peroxidase, superoxide dismutase, and catalase, which ameliorated subsequent embryo development and embryo quality (p < 0.05). Taken together, these results suggest that quercetin protects both goat sperm and preimplantation embryos from Cd²⁺-induced oxidative stress.
Agid:
6090554