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Protective Effects of Quercetin Against Cadmium Chloride-Induced Oxidative Injury in Goat Sperm and Zygotes

Mao, Tingchao, Han, Chengquan, Wei, Biao, Zhao, Lu, Zhang, Qing, Deng, Ruizhi, Liu, Jun, Luo, Yan, Zhang, Yong
Biological trace element research 2018 v.185 no.2 pp. 344-355
antioxidant activity, cadmium, catalase, embryogenesis, gene expression, glutathione peroxidase, goats, malondialdehyde, mitochondria, oxidative stress, peroxidation, protective effect, quercetin, reactive oxygen species, sperm quality, superoxide dismutase, survival rate, zygote
Quercetin, a plant-derived flavonoid, is frequently used as an antioxidant for efficient anti-oxidative capacity. However, whether quercetin has protective effects on goat sperm and preimplantation embryos against Cd²⁺-induced oxidative injury is still unclear. So, we researched the influence of quercetin on goat sperm and zygotes respectively under the oxidative stress induced by Cd²⁺. In our study, quercetin decreased the malonaldehyde (MDA) and reactive oxygen species (ROS) levels caused by Cd²⁺ in goat sperm (p < 0.05), which facilitated sperm characteristics including motility, survival rates, membrane integrity, and mitochondria activity during storage in vitro and subsequent embryo development (p < 0.05). Moreover, in goat zygotes, quercetin decreased peroxidation products including ROS, MDA, and carbonyl through preserving or maintaining mitochondrial function, gene expression, and anti-oxidative products such as glutathione peroxidase, superoxide dismutase, and catalase, which ameliorated subsequent embryo development and embryo quality (p < 0.05). Taken together, these results suggest that quercetin protects both goat sperm and preimplantation embryos from Cd²⁺-induced oxidative stress.