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Evaluation of cancer cell deformability by microcavity array

Author:
Yoshino, Tomoko, Tanaka, Tsuyoshi, Nakamura, Seita, Negishi, Ryo, Shionoiri, Nozomi, Hosokawa, Masahito, Matsunaga, Tadashi
Source:
Analytical biochemistry 2017 v.520 pp. 16-21
ISSN:
0003-2697
Subject:
breast neoplasms, confocal laser scanning microscopy, epithelium, filtration, human cell lines, humans, neoplasm cells, porosity
Abstract:
A cell entrapment device consisting of a microcavity array was used to analyze the deformability of MCF-10 human breast epithelial and MCF-7 human breast cancer cell lines by confocal laser scanning microscopy. Entrapment of up to 8 × 10³ cells was achieved within 3 min. Protrusions were formed at the bottom surface of the array with a pore size of 3 μm. Protrusion length increased at higher filtration pressures and could be used to distinguish between MCF-7 and MCF-10 cells. These results indicate that our system is useful for high-throughput deformability analysis of cancer cells, which can provide insight into the mechanisms underlying tumor cell malignancy.
Agid:
6092067